Titulo:
"Transcriptional profiling and genotyping in the era of next generation sequencing"

Resumen:
The advent of next generation sequencing (NGS), particularly the Illumina sequencing platforms, have revolutionized genome and transcriptome analyses. RNA and DNA sequencing are now, respectively, cost effective options for transcriptome analysis and for mapping mutations. We use RNA sequencing to study the dynamics of jasmonate signaling, and DNA sequencing to map mutations in tomato and arabidopsis. While NGS is becoming a routine tool for molecular biology, the generation and processing of massive amounts of sequencing data can be challenging and time consuming. However, these approaches offer numerous advantages over conventional methods. For example, RNA sequencing permits de novo analysis of whole transcriptomes without a reference genome, provides a higher dynamic range of detection and absolute transcript abundance, and can detect alternative splicing and novel isoforms. DNA sequencing can dramatically speed up the identification of causal mutations compared with conventional recombinant genotyping and candidate sequencing. In this presentation, I will describe the NGS workflow, with particular attention to data processing (currently the limiting step), as well as discuss some of the advantages and disadvantages of NGS using examples from our own work.

Organizado por: Instituto de Agrobiotecnología del Litoral.